Support Center
Providing revolutionary resolutions for life science research and IVD
Support
Q&A
Q: How to Install the Software
MobiVision®-M is a ready-to-use software package. Each time you open a new shell terminal, activate the environment by source to start using it.
Q: Requirements for Input Fastq Files
MobiVision®-M accepts paired-end sequencing data from the second generation as input files and can automatically merge data from multiple sequencing runs. For example:
MobiVision identifies the sequencing data in the following pattern:
（prefix）（flag）（postfix）（type）
Where:
The flag is used to determine whether the sequencing file is from R1 or R2. Currently supported flags are R1|R2|1|2.
The prefix and postfix are strings before and after the flag. If no id is specified, the prefix will be used as the id. The postfix can be empty.
The type is the file extension, and supported extensions include fastq|fq|fastq.gz|fq.gz.
MobiVision®-M will check each file in the input folder and treat files that match this pattern as data to be analyzed.
MobiVision®-M considers sequencing data with the same prefix+postfix+type as coming from the same sequencing run. If multiple runs are detected, the data from multiple runs will be merged in order and then analyzed.
Q: Do I Need to Merge Data from Multiple Sequencing Runs in Advance
No, MobiVision®-M can automatically merge data from multiple sequencing runs. See Question 1 for details.
Q: Differences Between raw_cell_gene_matrix and raw_re-assigned_cell_gene_matrix
The differences between the three output matrices are as follows:
Q: What to Do If the Number of Cells Is Too Low or the Median Gene Count Is Too Low
Generally, both situations can be fixed by specifying the number of cells.
Increasing the number of cells will decrease the median gene count, and vice versa. Adjust according to the actual sample conditions.
Q: Why There Is No Bam File Output
By default, MobiVision®-M deletes the bam files and the unmapped reads files.
If you need to retain these files, you need to add the --keep_bam (to keep the bam files) and --keep_unmap_reads (to keep the unmapped reads) options in the command.