The runtime for 100GB of data depends on the server configuration and parameter settings. For example, using a Hygon C86 7285H 32-core Processor (2.5GHz):

1. For samples around 10GB, increasing the number of threadsdoesn't significantly reduce analysis time but does increase memory usage.For 10GB libraries, 2-8 threads are recommended.

2. For 100GB samples, using fewer than 24 threads does notsignificantly increase memory usage but does reduce analysis time. Usingmore than 24 threads increases memory usage. For 100GB libraries, 16-24threads are recommended.

3. The runtime and memory consumption depend on the library sizeand the number of threads used. For a library size of 300GB, at least 64GBof memory is recommended for analysis.