For samples with around 30M reads, increasing the thread count significantly reduces analysis time when the thread count is below 8, without significantly increasing memory usage. For samples with around 30M reads, increasing the thread count above 8 does not significantly reduce analysis time but greatly increases memory usage. Therefore, 8 threads are recommended for libraries with around 30M reads. If resources are ample, 16 threads can be used. The memory usage and runtime depend on the library size and thread count. For libraries with 30M reads, 16GB of memory is recommended.